recombinant human her  (Sino Biological)

Journal: Biosensors

Article Title: Development of Electrochemical Immunosensors for HER-1 and HER-2 Analysis in Serum for Breast Cancer Patients

doi: 10.3390/bios13030355

Figure Lengend Snippet: Chronoamperometric signal for SPGE; ( a1 ) different concentrations of monoclonal anti-HER-1; ( a2 ) anti-HER-2 as the capture antibody; ( a3 ) the relative current percentage calculated for each concentration for both HER-1 and HER-2; ( b1 ) different concentrations of polyclonal anti-HER-1; ( b2 ) anti-HER-2 as the detector antibody; ( b3 ) the relative current percentage calculated for each concentration for both HER-1 and HER-2. Standard deviations were calculated for experiments performed in triplicate. In ( b1 , b2 ) CT stands for control readings.

Article Snippet: The active human HER-1 and HER-2 protein fragments, and the mouse anti-HER-2 monoclonal Ab were also purchased from Abcam (UK).

Techniques: Concentration Assay

Journal: Biosensors

Article Title: Development of Electrochemical Immunosensors for HER-1 and HER-2 Analysis in Serum for Breast Cancer Patients

doi: 10.3390/bios13030355

Figure Lengend Snippet: Biosensors responses of the optimized immunoassays against various concentrations of; ( a ) HER-1 antigen (0.5–75 ng mL −1 ); ( b ) HER-2 antigen (5–200 ng mL −1 ). Measurements were taken after 110 s at a potential of −200 mV. Embedded graphs show the raw data obtained. ( c ) Linear regression of the HER-1 and HER-2 assay, obtained with data taken from a,b. Standard deviations were calculated for experiments performed in triplicate ( n = 3).

Article Snippet: The active human HER-1 and HER-2 protein fragments, and the mouse anti-HER-2 monoclonal Ab were also purchased from Abcam (UK).

Techniques:

Journal: Biosensors

Article Title: Development of Electrochemical Immunosensors for HER-1 and HER-2 Analysis in Serum for Breast Cancer Patients

doi: 10.3390/bios13030355

Figure Lengend Snippet: The DLS results of the AuNP, before and after conjugation; ( a1 ), HER-1; ( a2 ), HER-2. Standard deviations were calculated for experiments performed in triplicate. ( b1 ), TEM imaging results of the gold nanoparticle before conjugation; ( b2 ), after conjugation with anti-rabbit -HRP; ( c ) HER-1, used here as an example of data achieved using different AuNP conjugate dilution ( n = 3).

Article Snippet: The active human HER-1 and HER-2 protein fragments, and the mouse anti-HER-2 monoclonal Ab were also purchased from Abcam (UK).

Techniques: Conjugation Assay, Imaging

Journal: Biosensors

Article Title: Development of Electrochemical Immunosensors for HER-1 and HER-2 Analysis in Serum for Breast Cancer Patients

doi: 10.3390/bios13030355

Figure Lengend Snippet: Biosensor response of the optimized immunoassay performed using AuNP-Ab-HRP conjugates against various concentrations of; ( a ) HER-1 antigen (0–0.5 ng mL −1 ) and ( b ) HER-2 antigen (0–50 ng mL −1 ). The embedded graphs show the raw data reading obtained from the chronoamperometry measurements. ( c ) Linear regression curves for HER-1 and HER-2 AuNP-Ab-HRP conjugate assay, obtained with data taken from a,b. Standard deviations were calculated for experiments performed in triplicates ( n = 3).

Article Snippet: The active human HER-1 and HER-2 protein fragments, and the mouse anti-HER-2 monoclonal Ab were also purchased from Abcam (UK).

Techniques:

Journal: Biosensors

Article Title: Development of Electrochemical Immunosensors for HER-1 and HER-2 Analysis in Serum for Breast Cancer Patients

doi: 10.3390/bios13030355

Figure Lengend Snippet: Serum samples concentrations (50, 75 and 100%) with no HER-1 and HER-2 antigen applied to the immunosensor assay. Standard deviation is for experiments performed in triplicates ( n = 3).

Article Snippet: The active human HER-1 and HER-2 protein fragments, and the mouse anti-HER-2 monoclonal Ab were also purchased from Abcam (UK).

Techniques: Standard Deviation

Journal: Biosensors

Article Title: Development of Electrochemical Immunosensors for HER-1 and HER-2 Analysis in Serum for Breast Cancer Patients

doi: 10.3390/bios13030355

Figure Lengend Snippet: Standardized biosensor response of the optimized immunoassay against increasing concentrations of ( a ) HER-1 antigen (0.5–75 ng mL −1 ); ( b ) HER-2 antigen (5–80 ng mL −1 ) spiked in 100% serum. Embedded graph shows the raw chronoamperometry measurements. ( c ) Linear regression of the assay spiked in serum samples with data taken from a,b. Standard deviations were calculated from experiments performed in triplicate ( n = 3).

Article Snippet: The active human HER-1 and HER-2 protein fragments, and the mouse anti-HER-2 monoclonal Ab were also purchased from Abcam (UK).

Techniques:

Journal: Biosensors

Article Title: Development of Electrochemical Immunosensors for HER-1 and HER-2 Analysis in Serum for Breast Cancer Patients

doi: 10.3390/bios13030355

Figure Lengend Snippet: ( a ) Standardized biosensor response of the optimized immunoassay against various concentrations of HER-2 antigen spiked in 100% serum (5–25 ng mL −1 ); the embedded graph shows the raw chronoamperometry measurements. ( b ) Linear regression of the AuNP-Ab-HRP conjugate assay spiked in serum samples obtained using data taken from a. ( c ) Cross-reactivity study of the HER-2 sensor for HER-1 via the indirect ELISA sandwich assay performed using AuNPs-Ab-HRP conjugate as a signal amplification in both buffer and 100% serum. Standard deviations were calculated for experiments performed in triplicate ( n = 3).

Article Snippet: The active human HER-1 and HER-2 protein fragments, and the mouse anti-HER-2 monoclonal Ab were also purchased from Abcam (UK).

Techniques: Indirect ELISA, Amplification

Journal: Scientific Reports

Article Title: Application of green synthesized WO 3 -poly glutamic acid nanobiocomposite for early stage biosensing of breast cancer using electrochemical approach

doi: 10.1038/s41598-021-03209-8

Figure Lengend Snippet: The concentration-electrochemical signals correlation using the proposed immunosensor. ( A ) the current–potential signals and ( B ) the obtained signals for different concentrations of the HER-2 protein (n = 2). The electrochemical measurements were obtained in a PBS buffer (pH = 7.4) of 5 mM K 4 [Fe(CN) 6 ] and 0.1 M KCl. All the DPV measurements were obtained in tha potential range of -0.1–0.6 V with pulse amplitude of 5 mV with interval time of 0.5 s.

Article Snippet: HER-2 antibody (Ab) and HER-2 protein were obtained from Abcam.

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Application of green synthesized WO 3 -poly glutamic acid nanobiocomposite for early stage biosensing of breast cancer using electrochemical approach

doi: 10.1038/s41598-021-03209-8

Figure Lengend Snippet: Comparison of the proposed biosensor with the other previously reported platforms for HER-2 protein.

Article Snippet: HER-2 antibody (Ab) and HER-2 protein were obtained from Abcam.

Techniques:

Journal: Scientific Reports

Article Title: Application of green synthesized WO 3 -poly glutamic acid nanobiocomposite for early stage biosensing of breast cancer using electrochemical approach

doi: 10.1038/s41598-021-03209-8

Figure Lengend Snippet: Analysis of a real sample of HER-2 positive patient and comparison of the correlated signals with a standard solution of HER-2 protein (1 ng/mL) and healthy real sample spiked with HER-2 protein (1 ng/mL). The electrochemical measurements were obtained in a PBS buffer (pH = 7.4) of 5 mM K 4 [Fe(CN) 6 ] and 0.1 M KCl. All the DPV measurements were obtained in tha potential range of -0.1–0.6 V with pulse amplitude of 5 mV with interval time of 0.5 s.

Article Snippet: HER-2 antibody (Ab) and HER-2 protein were obtained from Abcam.

Techniques: